banda transcan 2 2016ul

Title: MicroRNAs methylation and expression profiling for identification of breast cancer patients at high risk to develop distant metastases.


Project Coordinator:
Manel ESTELLER (Spain) Bellvitge Biomedical Research Institute (IDIBELL) L'Hospitalet de Llobregat, Barcelona


Project Partners:
Paola PARRELLA (Italy) Fondazione Casa Sollievo della Sofferenza - I.R.C.C.S., San Giovanni Rotondo (FG)
Ella EVRON (Israel)  Assaf Harofeh Medical Center, Zerifin


Project Abstract:
Breast cancer is the most common cancer in women and its mortality is primarily caused by metastatic spread to distant organs. Thus, it is necessary to identify and validate metastasis molecular biomarkers at the time of surgery to predict and monitor breast cancer progression.
MicroRNAs (miRNAs) are a class of small cellular RNAs that modulate gene expression at post-transcriptional level. They can act as oncogenes or tumor-supressor genes, playing a role in all steps of tumorigenesis. MiRNAs up or down-regulation (mainly by promoter DNA hypermethylation) and metastatic spread have been widely investigated. However, only small and retrospective studies have been completed in a clinical setting to date. Here, we propose to evaluate the potential role as predictive markers of several candidate miRNAs already linked to metastatic process (selected from in vitro and limited sample size studies). We will analyze the correlation of both expression and DNA methylation markers with distant metastasis free survival in a large retrospective cohort (n=352). The best candidate markers will be validated in two independent and extensive (n=500 and n=300) prospective cohorts. Additionally, miRNA biomarkers both in plasma and Circulating Tumor Cells will be evaluated for treatment monitoring and early metastases detection. Of note, as DNA methylation is potentially reversible and several investigations with anticancer treatments targeting miRNAs are currently ongoing, this proposal may also identify novel therapeutic targets for high risk breast cancer patients.
The consortium partners guarantee the feasibility and strength of the proposal. The coordinator, Dr Esteller, has a wide and successful experience in epigenetic research and in the coordination of European projects. The clinical teams have a solid background in breast cancer clinical and epigenetic studies. Altogether, the expertise of the partners and the possibility of using large retrospective, prospective and cross-validation cohorts may circumvent the limitations of the small studies completed to date in the search for miRNAs metastasis biomarkers.

Final Summary:
Breast cancer is the most common cancer in women and its mortality is primarily caused by metastatic spread to distant organs. Thus, it is necessary to identify and validate metastasis molecular biomarkers at the time of surgery to predict and monitor breast cancer progression. MicroRNAs (miRNAs) are a class of small noncoding RNAs and play a critical role in cell biology by regulating gene expression at the post-transcriptional level. They can act as oncogenes or tumor-suppressor genes, playing a role in all steps of tumorigenesis. Aberrant DNA methylation is an epigenetic transcriptional silencing mechanism that can be associated as one of the mechanisms of down-regulation of miRNAs, which is currently emerging as a common hallmark of cancer. Metastasis is the major cause of cancer mortality, and an important clinical problem that has been widely investigated. However, only small and retrospective studies have been completed in a clinical setting to date.

BREMIR’s research project is based on the identification of a panel of microRNAs as biomarkers that could be predictive of distant metastasis in breast cancer patients. In order to achieve this aim, we analyzed a panel of miRNAs by gene expression and DNA methylation analysis of breast cancer samples at early-stage of progression with low risk of relapse. We identified and select 11 miRNAs associated with disease progression and metastasis development. Next, a new set of miRNAs related to poor prognosis were selected and validated. The results are promising in overexpressed miRNAs as predictive biomarkers, opening the range of possibilities for a better clinical management of breast cancer patients.

Overall, we identified a new set of predictive miRNAs that determine the metastatic potential of breast cancer tumors and may ultimately help select patients more likely to develop breast cancer distant metastasis.

 

(Project funded under JTC 2013)


TRANSCAN News

 TRANSCAN-3 news on:
twitter logoinkedin social media logo

TRANSCAN-3   JOINT TRANSNATIONAL CALL 2022 on:
 "Novel translational approaches to tackle the challenges of hard-to-treat cancers from early diagnosis to therapy"  

is open for submission

Please visit the TRANSCAN-3 website at
https://transcan.eu/funding/calls/joint-call-jtc-2022-.kl


Other news:

The Digital Institute for Cancer Outcomes Research (DIGICORE),  is delighted to announce an innovative new training programme for early career researchers in oncology: IDEAL4RWE.
 Please register for an introductory webinar on the19th April 2022(https://lnkd.in/ez7y4kWU).
Brochure for more details: https://tinyurl.com/y7aurdtk


 The first TRANSCAN-3 call (JTC 2021) on:
"Next generation cancer immunotherapy: targeting the tumour microenvironment"
is closed for submission

International Networking Event (virtual)
on 22 April 2021 hrs 9:00 CET
will focus on the forthcoming co-funded JTC 2021 on:
"Next generation cancer immunotherapy: targeting the tumour microenvironment" 
the event is organized by TUBITAK under the “Turkey in Horizon 2020 Phase II” Technical Assistance Project
in cooperation with TRANSCAN-3

The new ERA-NET Cofund TRANSCAN-3:
 Sustained collaboration of national and regional programmes in cancer research
is funded by the European Commission under H2020  which started on 1st March 2021 and will last five years (G. A. no. 964264)

A TRANSCAN-2 brochure, highlighting key achievements, is available for download

 

Newsletters

The TRANSCAN-3 newsletters are published at: https://www.transcan.eu/

SUBSCRIBE to the newsletters
You can unsubscribe at any moment by clicking on the subscription preferences link at the bottom of a received newsletter


All the TRANSCAN-2 newsletters:

Newsletter 10, Sept.2020
Newsletter 9, Dec.2019

Newsletter 8, May 2019
Newsletter 7, Oct.2018
Newsletter 6, June 2108
Newsletter 5, March 2018
Newsletter 4, Dec. 2017
Newsletter 3, Sept. 2017
Newsletter 2, April 2017
Newsletter 1, Dec. 2016

eu flagship

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 643638.

Who's Online

We have 10 guests and no members online