banda transcan 2 2016ul

Title: Non-invasive prognostic markers for Resected Early-STage NSCLC: role of circulatING and exosomal miRNAs and free circulating DNA

Project Coordinator:
Paola ULIVI (Italy) IRCCS Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori IRST, Meldola

 
Project Partners:
Enriqueta FELIP (Spain) Vall d'Hebron Institute of Oncology, Barcelona
Elisabeth QUOIX (France) CHU de Strasbourg - Hôpital Civil, Strasbourg


Project Abstract:
Background and rationale: Non-small cell lung cancer (NSCLC) is the primary cause of cancer-related death. Worldwide, about 20% of cases are diagnosed at an early stage, allowing an effective pulmonary resection. However, lung cancer 5-years survival rate remains below 16% mainly because of disseminated disease, also in fully resected early stages. Biomarkers able to identify patients with a higher risk of relapse could be very useful. Circulating microRNAs (miRNAs), in both exosome-free and exosome-encapsulated forms, represent promising markers in this setting. Some studies in the literature and our preliminary data have shown that specific miRNA signatures could have a prognostic role in NSCLC, both in the early-stage and in the metastatic disease. Moreover, free circulating DNA (fcDNA) is another prognostic marker in advanced NSCLC, whereas its role in earlystage lung cancer is not so clear yet.
Hypothesis: We hypothesize that exosomal and/or circulating miRNAs could represent prognostic biomarkers in resected-early-stage NSCLC and that a different expression profile at baseline, before surgery, could be indicative of a different patient survival. We also hypothesize that fcDNA could also be, singly or in combination with exosomal/circulating miRNAs, a prognostic biomarker in this setting.
Aims: Primary:
- To evaluate the prognostic value of exosomal and cell-free miRNAs in relation to disease free survival (DFS) and overall survival (OS) in a prospective case series of resected early-stage (stages IA-IIIA) NSCLC patients.
Secondary:
- To analyze the role of fcDNA in relation to DFS and OS
- To analyze the association between exosomal and cell-free miRNAs and fcDNA and their combinations in relation to patient outcome
Methods: Two peripheral blood samples will be collected from each of the 220 expected patients (stage IA-IIIA), before the surgical resection. Circulating miRNAs will be extracted from serum using specific kits. Isolation of plasma exosomes and exosome-associated miRNA extraction will be performed using specific commercial kits. Free circulating DNA will be extracted from plasma and quantified by a Real Time PCR method.
Expected results and potential impact:
We expect to evaluate the prognostic role of exosomal and cell-free miRNA and fcDNA for resected early-stage NSCLC patients. We will study these markers singly or in combination, for their capacity to predict patients with a high risk of relapse and poor survival with respect to those with a better prognosis. The use of non-invasive biomarkers in this setting could be very useful in the clinical practice for a better patient management.

 

 

(Project funded under JTC 2016)


TRANSCAN News

Launch of the call: JTC 2021
The new TRANSCAN-3 project consortium is launching the first Joint Transnational Call for proposals, co-funded by the European Commission, on:
"Next generation cancer immunotherapy: targeting the tumour microenvironment"

International Networking Event (virtual)
on 22 April 2021 hrs 9:00 CET
will focus on the forthcoming co-funded JTC 2021 on:
"Next generation cancer immunotherapy: targeting the tumour microenvironment" 
the event is organized by TUBITAK under the “Turkey in Horizon 2020 Phase II” Technical Assistance Project
in cooperation with TRANSCAN-3

ERA-NET Cofund TRANSCAN-3:
 Sustained collaboration of national and regional programmes in cancer research
is the new project funded by the European Commission under H2020  which started on 1st March 2021 and will last five years (G. A. no. 964264)

A TRANSCAN-2 brochure, highlighting key achievements, is available for download

 

eu flagship

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 643638.

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